Biotinylation is the process of covalently attaching biotin to a biomolecular platform such as a protein, sugar, nucleic acid, or others. Biotin, itself, binds with exceptional affinity (KD ~ 10-15 - 10-14) to three related proteins (avidin, streptavidin, and neutravidin). The biotin-avidin interaction is commonly exploited in biotechnology for applications ranging from biomolecular purification, and labeling. The rate of complex formation for the biotin-avidin interaction is rapid, and once formed is highly stable to a variety of buffer conditions.
NuLink Biotin Reagents
Fast Reliable Results
Dual Monitoring with either U.V. or Fluorescence
Classical reagents used for biotinylation exhibit poor water solubility thereby necessitating the use of organic co-solvents such as DMF, DMSO or acetonitrile. Because the use of such organic solvents is often contraindicated in many biotechnology applications it is highly desirable that the chosen applications be compatible with aqueous systems. NuChemie's NuLink Biotin reagents all exhibit excellent water solubility, ranging in solubilities from 2.5 - 20 mg/ml.
NuLink Biotin reagents offer additional benefits over other classical biotinylation reagents. Like all reagents within the NuLink platform, Nulink Biotin reagents are easily quantified using a simple U.V. scan. This attribute of the NulInk Biotin platform renders the use of the standard secondary HABA assay unnecessary.
Like the rest of the NuLink platform NuChemie's NuLink Biotin reagents offer exceptional characteristics over standard N-hydroxysuccinimide (NHS) esters. The NuLink platform exhibits superior stability under aqueous conditions, whilst concurrently exhibiting faster reaction kinetics with primary amines compared to similar NHS systems.
NuLink Biotin-15 | 15-Angstrom Spacer | SKU NCB015 | 5 mg, 5 x 1 mg Click to Buy
NuLink Biotin-21 | 21-Angstrom Spacer | SKU NCB021 | 5 mg, 5 x 1 mg Click to Buy
NuLink Biotin-24 | 24-Angstrom Spacer | SKU NCB024 | 5 mg, 5 x 1 mg Click to Buy
NuLink Biotin-33 | 33-Angstrom Spacer | SKU NCB033 | 5 mg, 5 x 1 mg Click to Buy
NuLink Biotin Kit Sets | UV Traceable Click to Browse
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